Review article
Linear Regression Analysis
Part 14 of a Series on Evaluation of Scientific Publications
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Background: Regression analysis is an important statistical method for the analysis of medical data. It enables the identification and characterization of relationships among multiple factors. It also enables the identification of prognostically relevant risk factors and the calculation of risk scores for individual prognostication.
Methods: This article is based on selected textbooks of statistics, a selective review of the literature, and our own experience.
Results: After a brief introduction of the uni and multivariable regression models, illustrative examples are given to explain what the important considerations are before a regression analysis is performed, and how the results should be interpreted. The reader should then be able to judge whether the method has been used correctly and interpret the results appropriately.
Conclusion: The performance and interpretation of linear regression analysis are subject to a variety of pitfalls, which are discussed here in detail. The reader is made aware of common errors of interpretation through practical examples. Both the opportunities for applying linear regression analysis and its limitations are presented.
The purpose of statistical evaluation of medical data is often to describe relationships between two variables or among several variables. For example, one would like to know not just whether patients have high blood pressure, but also whether the likelihood of having high blood pressure is influenced by factors such as age and weight. The variable to be explained (blood pressure) is called the dependent variable, or, alternatively, the response variable; the variables that explain it (age, weight) are called independent variables or predictor variables. Measures of association provide an initial impression of the extent of statistical dependence between variables. If the dependent and independent variables are continuous, as is the case for blood pressure and weight, then a correlation coefficient can be calculated as a measure of the strength of the relationship between them (Box 1 gif ppt).
Regression analysis is a type of statistical evaluation that enables three things:
 Description: Relationships among the dependent variables and the independent variables can be statistically described by means of regression analysis.
 Estimation: The values of the dependent variables can be estimated from the observed values of the independent variables.
 Prognostication: Risk factors that influence the outcome can be identified, and individual prognoses can be determined.
Regression analysis employs a model that describes the relationships between the dependent variables and the independent variables in a simplified mathematical form. There may be biological reasons to expect a priori that a certain type of mathematical function will best describe such a relationship, or simple assumptions have to be made that this is the case (e.g., that blood pressure rises linearly with age). The bestknown types of regression analysis are the following (Table 1 gif ppt):
 Linear regression,
 Logistic regression, and
 Cox regression.
The goal of this article is to introduce the reader to linear regression. The theory is briefly explained, and the interpretation of statistical parameters is illustrated with examples. The methods of regression analysis are comprehensively discussed in many standard textbooks (1–3).
Cox regression will be discussed in a later article in this journal.
Methods
Linear regression is used to study the linear relationship between a dependent variable Y (blood pressure) and one or more independent variables X (age, weight, sex).
The dependent variable Y must be continuous, while the independent variables may be either continuous (age), binary (sex), or categorical (social status). The initial judgment of a possible relationship between two continuous variables should always be made on the basis of a scatter plot (scatter graph). This type of plot will show whether the relationship is linear (Figure 1 gif ppt) or nonlinear (Figure 2 gif ppt).
Performing a linear regression makes sense only if the relationship is linear. Other methods must be used to study nonlinear relationships. The variable transformations and other, more complex techniques that can be used for this purpose will not be discussed in this article.
Univariable linear regression
Univariable linear regression studies the linear relationship between the dependent variable Y and a single independent variable X. The linear regression model describes the dependent variable with a straight line that is defined by the equation Y = a + b × X, where a is the yintersect of the line, and b is its slope. First, the parameters a and b of the regression line are estimated from the values of the dependent variable Y and the independent variable X with the aid of statistical methods. The regression line enables one to predict the value of the dependent variable Y from that of the independent variable X. Thus, for example, after a linear regression has been performed, one would be able to estimate a person’s weight (dependent variable) from his or her height (independent variable) (Figure 3 gif ppt).
The slope b of the regression line is called the regression coefficient. It provides a measure of the contribution of the independent variable X toward explaining the dependent variable Y. If the independent variable is continuous (e.g., body height in centimeters), then the regression coefficient represents the change in the dependent variable (body weight in kilograms) per unit of change in the independent variable (body height in centimeters). The proper interpretation of the regression coefficient thus requires attention to the units of measurement. The following example should make this relationship clear:
In a fictitious study, data were obtained from 135 women and men aged 18 to 27. Their height ranged from 1.59 to 1.93 meters. The relationship between height and weight was studied: weight in kilograms was the dependent variable that was to be estimated from the independent variable, height in centimeters. On the basis of the data, the following regression line was determined: Y= –133.18 + 1.16 × X, where X is height in centimeters and Y is weight in kilograms. The yintersect a = –133.18 is the value of the dependent variable when X = 0, but X cannot possibly take on the value 0 in this study (one obviously cannot expect a person of height 0 centimeters to weigh negative 133.18 kilograms). Therefore, interpretation of the constant is often not useful. In general, only values within the range of observations of the independent variables should be used in a linear regression model; prediction of the value of the dependent variable becomes increasingly inaccurate the further one goes outside this range.
The regression coefficient of 1.16 means that, in this model, a person’s weight increases by 1.16 kg with each additional centimeter of height. If height had been measured in meters, rather than in centimeters, the regression coefficient b would have been 115.91 instead. The constant a, in contrast, is independent of the unit chosen to express the independent variables. Proper interpretation thus requires that the regression coefficient should be considered together with the units of all of the involved variables. Special attention to this issue is needed when publications from different countries use different units to express the same variables (e.g., feet and inches vs. centimeters, or pounds vs. kilograms).
Figure 3 shows the regression line that represents the linear relationship between height and weight.
For a person whose height is 1.74 m, the predicted weight is 68.50 kg (y = –133.18 + 115.91 × 1.74 m). The data set contains 6 persons whose height is 1.74 m, and their weights vary from 63 to 75 kg.
Linear regression can be used to estimate the weight of any persons whose height lies within the observed range (1.59 m to 1.93 m). The data set need not include any person with this precise height. Mathematically it is possible to estimate the weight of a person whose height is outside the range of values observed in the study. However, such an extrapolation is generally not useful.
If the independent variables are categorical or binary, then the regression coefficient must be interpreted in reference to the numerical encoding of these variables. Binary variables should generally be encoded with two consecutive whole numbers (usually 0/1 or 1/2). In interpreting the regression coefficient, one should recall which category of the independent variable is represented by the higher number (e.g., 2, when the encoding is 1/2). The regression coefficient reflects the change in the dependent variable that corresponds to a change in the independent variable from 1 to 2.
For example, if one studies the relationship between sex and weight, one obtains the regression line Y = 47.64 + 14.93 × X, where X = sex (1 = female, 2 = male). The regression coefficient of 14.93 reflects the fact that men are an average of 14.93 kg heavier than women.
When categorical variables are used, the reference category should be defined first, and all other categories are to be considered in relation to this category.
The coefficient of determination, r^{2}, is a measure of how well the regression model describes the observed data (Box 2 gif ppt). In univariable regression analysis, r^{2} is simply the square of Pearson’s correlation coefficient. In the particular fictitious case that is described above, the coefficient of determination for the relationship between height and weight is 0.785. This means that 78.5% of the variance in weight is due to height. The remaining 21.5% is due to individual variation and might be explained by other factors that were not taken into account in the analysis, such as eating habits, exercise, sex, or age.
In formal terms, the null hypothesis, which is the hypothesis that b = 0 (no relationship between variables, the regression coefficient is therefore 0), can be tested with a ttest. One can also compute the 95% confidence interval for the regression coefficient (4).
Multivariable linear regression
In many cases, the contribution of a single independent variable does not alone suffice to explain the dependent variable Y. If this is so, one can perform a multivariable linear regression to study the effect of multiple variables on the dependent variable.
In the multivariable regression model, the dependent variable is described as a linear function of the independent variables X_{i}, as follows: Y = a + b1 × X1 + b2 × X_{2 }+…+ b_{n }× X_{n} . The model permits the computation of a regression coefficient b_{i} for each independent variable X_{i} (Box 3 gif ppt).
Just as in univariable regression, the coefficient of determination describes the overall relationship between the independent variables X_{i} (weight, age, bodymass index) and the dependent variable Y (blood pressure). It corresponds to the square of the multiple correlation coefficient, which is the correlation between Y and b_{1 }× X_{1} + ... + b_{n }× X_{n}.
It is better practice, however, to give the corrected coefficient of determination, as discussed in Box 2. Each of the coefficients b_{i} reflects the effect of the corresponding individual independent variable X_{i} on Y, where the potential influences of the remaining independent variables on X_{i }have been taken into account, i.e., eliminated by an additional computation. Thus, in a multiple regression analysis with age and sex as independent variables and weight as the dependent variable, the adjusted regression coefficient for sex represents the amount of variation in weight that is due to sex alone, after age has been taken into account. This is done by a computation that adjusts for age, so that the effect of sex is not confounded by a simultaneously operative age effect (Box 4 gif ppt).
In this way, multivariable regression analysis permits the study of multiple independent variables at the same time, with adjustment of their regression coefficients for possible confounding effects between variables.
Multivariable analysis does more than describe a statistical relationship; it also permits individual prognostication and the evaluation of the state of health of a given patient. A linear regression model can be used, for instance, to determine the optimal values for respiratory function tests depending on a person’s age, bodymass index (BMI), and sex. Comparing a patient’s measured respiratory function with these computed optimal values yields a measure of his or her state of health.
Medical questions often involve the effect of a very large number of factors (independent variables). The goal of statistical analysis is to find out which of these factors truly have an effect on the dependent variable. The art of statistical evaluation lies in finding the variables that best explain the dependent variable.
One way to carry out a multivariable regression is to include all potentially relevant independent variables in the model (complete model). The problem with this method is that the number of observations that can practically be made is often less than the model requires. In general, the number of observations should be at least 20 times greater than the number of variables under study.
Moreover, if too many irrelevant variables are included in the model, overadjustment is likely to be the result: that is, some of the irrelevant independent variables will be found to have an apparent effect, purely by chance. The inclusion of irrelevant independent variables in the model will indeed allow a better fit with the data set under study, but, because of random effects, the findings will not generally be applicable outside of this data set (1). The inclusion of irrelevant independent variables also strongly distorts the determination coefficient, so that it no longer provides a useful index of the quality of fit between the model and the data (Box 2).
In the following sections, we will discuss how these problems can be circumvented.
The selection of variables
For the regression model to be robust and to explain Y as well as possible, it should include only independent variables that explain a large portion of the variance in Y. Variable selection can be performed so that only such independent variables are included (1).
Variable selection should be carried out on the basis of medical expert knowledge and a good understanding of biometrics. This is optimally done as a collaborative effort of the physicianresearcher and the statistician. There are various methods of selecting variables:
Forward selection
Forward selection is a stepwise procedure that includes variables in the model as long as they make an additional contribution toward explaining Y. This is done iteratively until there are no variables left that make any appreciable contribution to Y.
Backward selection
Backward selection, on the other hand, starts with a model that contains all potentially relevant independent variables. The variable whose removal worsens the prediction of the independent variable of the overall set of independent variables to the least extent is then removed from the model. This procedure is iterated until no dependent variables are left that can be removed without markedly worsening the prediction of the independent variable.
Stepwise selection
Stepwise selection combines certain aspects of forward and backward selection. Like forward selection, it begins with a null model, adds the single independent variable that makes the greatest contribution toward explaining the dependent variable, and then iterates the process. Additionally, a check is performed after each such step to see whether one of the variables has now become irrelevant because of its relationship to the other variables. If so, this variable is removed.
Block inclusion
There are often variables that should be included in the model in any case—for example, the effect of a certain form of treatment, or independent variables that have already been found to be relevant in prior studies. One way of taking such variables into account is their block inclusion into the model. In this way, one can combine the forced inclusion of some variables with the selective inclusion of further independent variables that turn out to be relevant to the explanation of variation in the dependent variable.
The evaluation of a regression model requires the performance of both forward and backward selection of variables. If these two procedures result in the selection of the same set of variables, then the model can be considered robust. If not, a statistician should be consulted for further advice.
Discussion
The study of relationships between variables and the generation of risk scores are very important elements of medical research. The proper performance of regression analysis requires that a number of important factors should be considered and tested:
1. Causality
Before a regression analysis is performed, the causal relationships among the variables to be considered must be examined from the point of view of their content and/or temporal relationship. The fact that an independent variable turns out to be significant says nothing about causality. This is an especially relevant point with respect to observational studies (5).
2. Planning of sample size
The number of cases needed for a regression analysis depends on the number of independent variables and of their expected effects (strength of relationships). If the sample is too small, only very strong relationships will be demonstrable. The sample size can be planned in the light of the researchers’ expectations regarding the coefficient of determination (r^{2}) and the regression coefficient (b). Furthermore, at least 20 times as many observations should be made as there are independent variables to be studied; thus, if one wants to study 2 independent variables, one should make at least 40 observations.
3. Missing values
Missing values are a common problem in medical data. Whenever the value of either a dependent or an independent variable is missing, this particular observation has to be excluded from the regression analysis. If many values are missing from the dataset, the effective sample size will be appreciably diminished, and the sample may then turn out to be too small to yield significant findings, despite seemingly adequate advance planning. If this happens, real relationships can be overlooked, and the study findings may not be generally applicable. Moreover, selection effects can be expected in such cases. There are a number of ways to deal with the problem of missing values (6).
4. The data sample
A further important point to be considered is the composition of the study population. If there are subpopulations within it that behave differently with respect to the independent variables in question, then a real effect (or the lack of an effect) may be masked from the analysis and remain undetected. Suppose, for instance, that one wishes to study the effect of sex on weight, in a study population consisting half of children under age 8 and half of adults. Linear regression analysis over the entire population reveals an effect of sex on weight. If, however, a subgroup analysis is performed in which children and adults are considered separately, an effect of sex on weight is seen only in adults, and not in children. Subgroup analysis should only be performed if the subgroups have been predefined, and the questions already formulated, before the data analysis begins; furthermore, multiple testing should be taken into account (7, 8).
5. The selection of variables
If multiple independent variables are considered in a multivariable regression, some of these may turn out to be interdependent. An independent variable that would be found to have a strong effect in a univariable regression model might not turn out to have any appreciable effect in a multivariable regression with variable selection. This will happen if this particular variable itself depends so strongly on the other independent variables that it makes no additional contribution toward explaining the dependent variable. For related reasons, when the independent variables are mutually dependent, different independent variables might end up being included in the model depending on the particular technique that is used for variable selection.
Overview
Linear regression is an important tool for statistical analysis. Its broad spectrum of uses includes relationship description, estimation, and prognostication. The technique has many applications, but it also has prerequisites and limitations that must always be considered in the interpretation of findings (Box 5 gif ppt).
Conflict of interest statement
The authors declare that they have no conflict of interest as defined by the guidelines of the International Committee of Medical Journal Editors.
Manuscript submitted on 11 May 2010, revised version accepted on 14 July 2010.
Translated from the original German by Ethan Taub, MD
Corresponding author
Prof. Dr. rer. nat. Maria Blettner
Department of Medical Biometrics, Epidemiology, and Computer Sciences
Johannes Gutenberg University
Obere Zahlbacher Str. 69
55131 Mainz
Germany
Prof. Dr. rer. nat. Blettner
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2.  Bortz J: Statistik für Humanund Sozialwissenschaftler. 6th edition. Heidelberg: Springer 2004. 
3.  Selvin S: Epidemiologic Analysis. Oxford University Press 2001. 
4.  Bender R, Lange S: Was ist ein Konfidenzintervall? Dtsch Med Wschr 2001; 126: T41. 
5.  Sir Bradford Hill A: The environment and disease: Association or Causation? Proc R Soc Med 1965; 58: 295–300. MEDLINE 
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7.  EMEA: Poiints to consider on multiplicity issues in clinical trials. 
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